Tutorial

Hint

Installation on Linux

Click here for installation help

  1. Download IMPI for Linux (Download)

  2. Download testdata (Download) (primers and reference gene sequence are also provided here)

  3. Install the third-party tools:

sudo apt-get install bowtie2
pip install umi_tools

  1. Download third-party tool NGmerge (Third party software)

Hint

Installation on Windows

Click here for installation help

  1. Download IMPI for Windows (Download)

  2. Download testdata (Download) (primers and reference gene sequence are also provided here)

  3. Download and install the third-party tools (Third party software)

Hint

NGmerge installation

Click here for installation help

Installation on Windows:

  1. Download & install cygwin: https://www.cygwin.com/setup-x86_64.exe

  2. Choose cygwin.mirror.constant

  3. Open cigwin and search and install the following versions:

    • gcc-core

    • make

    • cygwin32-zlib

    • openmpi

  4. Set as environment variable: C:\cygwin64\bin

  5. Run “make” in downloaded NGmerge folder

Installation on Linux

  1. Install

sudo apt-get install make gcc zlib-dev libomp-dev

  1. Run “make” in the downloaded NGmerge folder